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Identification of cadmium-induced genes in maize seedlings by suppression subtractive hybridization

Quanlin DAI, Baifei HUANG, Zhongyi YANG, Jiangang YUAN, Junzhi YANG

Frontiers of Environmental Science & Engineering 2010, Volume 4, Issue 4,   Pages 449-458 doi: 10.1007/s11783-010-0250-x

Abstract: In this paper, the technique of suppression subtractive hybridization (SSH) was used to isolate and identify

Keywords： cadmium (Cd)     suppression subtractive hybridization    

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Electromagnetic interference modeling and suppression techniques in variable-frequency drive systems

Le YANG, Shuo WANG, Jianghua FENG

Frontiers of Mechanical Engineering 2018, Volume 13, Issue 3,   Pages 329-353 doi: 10.1007/s11465-018-0466-1

Abstract: EMI noise suppression techniques, including passive filter, Wheatstone bridge balance, active filter,

Keywords： variable-frequency drive (VFD)     electromagnetic interference (EMI)     motor drive modeling     EMI noise suppression    

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Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 1,   Pages 121-122 doi: 10.15302/J-FASE-2017142

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Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 1,   Pages 48-58 doi: 10.15302/J-FASE-2016114

Abstract: Wheat stripe rust caused by f. sp. is one of the most devastating diseases of wheat worldwide and resistant cultivars are vital for its management. Therefore, investigating the heterozygosity of the pathogen is important because of rapid virulence changes in isolates heterozygous for avirulence/virulence. An isolate of f. sp. was selfed on to determine the heterozygosity for avirulence/virulence loci. One hundred and twenty progeny isolates obtained from this selfing were phenotyped using 25 lines of wheat containing genes and genotyped with 96 simple sequencing repeat markers, with 51 pathotypes and 55 multi-locus genotypes being identified. All of these were avirulent on lines with , , , and and virulent on lines with , and , indicating that the parental isolate was homozygously avirulent or homozygously virulent for these loci. Segregation was found for wheat lines with , , , , , , , , , , , , , , , and . The 17 cultivars to which the was identified as heterozygous with respect to virulence/avirulence should not be given priority in breeding programs to obtain new resistant cultivars.

Keywords： Puccinia striiformis f. sp. tritici     selfing     heterozygosity     virulence inheritance    

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Vibration suppression of speed-controlled robots with nonlinear control

Paolo BOSCARIOL,Alessandro GASPARETTO

Frontiers of Mechanical Engineering 2016, Volume 11, Issue 2,   Pages 204-212 doi: 10.1007/s11465-016-0380-3

Abstract: The capability of achieving improved trajectory tracking and vibration suppression is shown through experimental

Keywords： industrial robot     nonlinear control     vibration damping     model-free control     motion control    

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Effect of fiber hybridization on energy absorption and synergy in concrete

Ahmadreza RAMEZANI, Mohammad Reza ESFAHANI

Frontiers of Structural and Civil Engineering 2019, Volume 13, Issue 6,   Pages 1338-1349 doi: 10.1007/s17009-019-0558-2

Abstract: main concentration being on energy absorption characteristics of concrete and the efficacy of fiber hybridization

Keywords： hybrid fiber-reinforced concrete     synergy     toughness     steel fibers     polypropylene fibers    

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Study on concoction and water mist fire suppression performance of new composite additives

Liu Jianghong,Jin Xiang,Liao Guangxuan

Strategic Study of CAE 2010, Volume 12, Issue 9,   Pages 58-62

Abstract:

Water mists for fire suppression and control have widely received considerableA new composite additive was concocted and its effectiveness to water mist's fire suppression performanceResults showed that this additive can greatly improve the fire suppression efficiency of water mist andThe fire suppression mechanism of water mist with this additive was combined with physical and chemicalHowever, the surface cooling and fuel isolation were considered to be the crucial suppression mechanism

Keywords： water mist     additive     concoction     fire suppression performance    

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A convolutional neural network based approach to sea clutter suppression for small boat detection

Guan-qing Li, Zhi-yong Song, Qiang Fu,liguanqing09@nudt.edu.cn,songzhiyong08@nudt.edu.cn

Frontiers of Information Technology & Electronic Engineering 2020, Volume 21, Issue 10,   Pages 1413-1534 doi: 10.1631/FITEE.1900523

Abstract: In this paper we propose a novel convolutional neural network based dual-activated clutter suppressionSecond, we obtain the suppression coefficients by mapping the CAM inversely to the sea clutter spectrum
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Improvement of engine performance with high compression ratio based on knock suppression using Miller

Haiqiao WEI, Jie YU, Lei ZHOU

Frontiers in Energy 2019, Volume 13, Issue 4,   Pages 691-706 doi: 10.1007/s11708-019-0621-3

Abstract: In theory, high compression ratio has the potential to improve the thermal efficiency and promote the power output of the SI engine. However, the application of high compression ratio is substantially limited by the knock in practical working process. The objective of this work is to comprehensively investigate the application of high compression ratio on a gasoline engine based on the Miller cycle with boost pressure and split injection. In this work, the specific optimum strategies for CR10 and CR12 were experimentally investigated respectively on a single cylinder DISI engine. It was found that a high level of Miller cycle with a higher boost pressure could be used in CR12 to achieve an effective compression ratio similar to CR10, which could eliminate the knock limits at a high compression ratio and high load. To verify the advantages of the high compression ratio, the fuel economy and power performance of CR10 and CR12 were compared at full and partial loads. The result revealed that, compared with CR10, a similar power performance and a reduced fuel consumption of CR12 at full load could be achieved by using the strong Miller cycle and split injection. At partial load, the conditions of CR12 had very superior fuel economy and power performance compared to those of CR10.

Keywords： high compression ratio     knock     Miller cycle     split injection     engine performance    

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Growth suppression of colorectal cancer expressing S492R EGFR by monoclonal antibody CH12

Qiongna Dong, Bizhi Shi, Min Zhou, Huiping Gao, Xiaoying Luo, Zonghai Li, Hua Jiang

Frontiers of Medicine 2019, Volume 13, Issue 1,   Pages 83-93 doi: 10.1007/s11684-019-0682-z

Abstract: Furthermore, mAb CH12 showed significantly stronger growth suppression activities and induced a more

Keywords： S492R EGFR ectodomain mutation     colorectal cancer     mAb CH12     immunnotherapy    

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Fault tolerant control strategy for modular PWM current source inverter

Frontiers in Energy 2023, Volume 17, Issue 2,   Pages 228-238 doi: 10.1007/s11708-022-0852-6

Abstract: In this paper, a fault-tolerant control method for an input-series output-parallel modular grid-tied pulse-width modulation (PWM) current source inverter is proposed to address the most commonly seen single symmetrical gate-commutated thyristor (SGCT) open-circuit fault problems. This method actively offsets the neutral point of the current space vector to ensure a sinusoidal output of the grid current, and it can achieve the upper limit power of the inverter under the condition of a single SGCT open-circuit fault. In addition, an active damping control method based on grid harmonic current feedback is proposed after analyzing the influence of the transformer ferromagnetic resonance caused by the neutral point offset on the power quality of the grid current. It has been demonstrated that the proposed method effectively suppresses the resonance caused by the transformer and the modified modulation, improving the grid current’s power quality.

Keywords： source converter (CSC)     fault-tolerant control     space vector modulation     active damping     resonance suppression    

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Potential unreliability of ALK variant allele frequency in the efficacy prediction of targeted therapy in NSCLC

Frontiers of Medicine 2023, Volume 17, Issue 3,   Pages 493-502 doi: 10.1007/s11684-022-0946-x

Abstract: Anaplastic lymphoma kinase (ALK) is the most common fusion gene involved in non-small cell lung cancer (NSCLC), and remarkable response has been achieved with the use of ALK tyrosine kinase inhibitors (ALK-TKIs). However, the clinical efficacy is highly variable. Pre-existing intratumoral heterogeneity (ITH) has been proven to contribute to the poor treatment response and the resistance to targeted therapies. In this work, we investigated whether the variant allele frequencies (VAFs) of ALK fusions can help assess ITH and predict targeted therapy efficacy. Through the application of next-generation sequencing (NGS), 7.2% (326/4548) of patients were detected to be ALK positive. On the basis of the adjusted VAF (adjVAF, VAF normalization for tumor purity) of four different threshold values (adjVAF < 50%, 40%, 30%, or 20%), the association of ALK subclonality with crizotinib efficacy was assessed. Nonetheless, no statistical association was observed between median progression-free survival (PFS) and ALK subclonality assessed by adjVAF, and a poor correlation of adjVAF with PFS was found among the 85 patients who received first-line crizotinib. Results suggest that the ALK VAF determined by hybrid capture-based NGS is probably unreliable for ITH assessment and targeted therapy efficacy prediction in NSCLC.

Keywords： ALK fusion     next-generation sequencing     fluorescence in situ hybridization     immunohistochemistry    

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Engineering System of Weather Modification in China

He Zhijin,Wang Guanghe,Wang Yuzeng

Strategic Study of CAE 2000, Volume 2, Issue 7,   Pages 87-91

Abstract:

Weather modification (rain enhancement, hail suppression, fog clearing, etc.) is introduced in this

Keywords： weather modification     precipitation enhancement     hail suppression     fog clearing     engineering system    

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Self-assembly of metal-cholesterol oxidase hybrid nanostructures and application in bioconversion of steroids derivatives

Yu Xin, Qiuyue Gao, Yu Gu, Mengyao Hao, Guangming Fan, Liang Zhang

Frontiers of Chemical Science and Engineering 2021, Volume 15, Issue 3,   Pages 615-629 doi: 10.1007/s11705-020-1989-7

Abstract: electron microscopy assays, the metal-COD complexes revealed flower-like or granular structures after hybridizationHybridization in metal-based nanostructures could significantly enhance the initial conversion ratio

Keywords： cholesterol oxidase     metal-enzyme hybridization     nanostructures     sterol derivatives     bioconversion    

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UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY CROSSES WITH ‘ORAH’ MANDARIN (CITRUS RETICULATA)

Frontiers of Agricultural Science and Engineering 2021, Volume 8, Issue 2,

Abstract:

Seedless fruits are desirable in the citrus fresh fruit market. Triploid production via diploid × tetraploid interploidy crosses is thought to be the most efficient and widely-used strategy for the breeding of seedless citrus. Although ‘Orah’ mandarin has desirable organoleptic qualities, seeds in the fruits weaken its market competitiveness. To produce new seedless cultivars that are similar to ‘Orah’ mandarin, we performed three 2x × 4x crosses using ‘Orah’ mandarin as the seed parent to regenerate triploid plantlets. A total of 182 triploid and 36 tetraploid plantlets were obtained. By analyzing their genetic origins using nine novel single nucleotide polymorphism (SNP) markers, all of the triploids and tetraploids derived from these three crosses were proven to be hybrids. Also, we demonstrated that 2n megagametophyte formation in ‘Orah’ mandarin result in tetraploid production in these three interploidy crosses. These tetraploid plantlets were genotyped using eight pericentromeric SNP markers and nine centromere distal SNP markers. Based on the genotypes of the 2n megagametophytes, the parental heterozygosity rates in 16 SNP loci and all 2n megagametophytes were less than 50%, indicating that second division restitution was the mechanism underlying 2n megagametophyte formation at both the population and individual levels. These triploid hybrids enrich the germplasm available for seedless breeding. Moreover, the tetraploid hybrids are valuable as parents for ploidy breeding for the production of seedless citrus fruits.

 

Keywords： Citrus / 2n gamete / interploidy hybridization / pericentromeric SNP marker / second division restitution    

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Title Author Date Type Operation

Identification of cadmium-induced genes in maize seedlings by suppression subtractive hybridization

Quanlin DAI, Baifei HUANG, Zhongyi YANG, Jiangang YUAN, Junzhi YANG

Journal Article

Electromagnetic interference modeling and suppression techniques in variable-frequency drive systems

Le YANG, Shuo WANG, Jianghua FENG

Journal Article

Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

Journal Article

Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

Journal Article

Vibration suppression of speed-controlled robots with nonlinear control

Paolo BOSCARIOL,Alessandro GASPARETTO

Journal Article

Effect of fiber hybridization on energy absorption and synergy in concrete

Ahmadreza RAMEZANI, Mohammad Reza ESFAHANI

Journal Article

Study on concoction and water mist fire suppression performance of new composite additives

Liu Jianghong,Jin Xiang,Liao Guangxuan

Journal Article

A convolutional neural network based approach to sea clutter suppression for small boat detection

Guan-qing Li, Zhi-yong Song, Qiang Fu,liguanqing09@nudt.edu.cn,songzhiyong08@nudt.edu.cn

Journal Article

Improvement of engine performance with high compression ratio based on knock suppression using Miller

Haiqiao WEI, Jie YU, Lei ZHOU

Journal Article

Growth suppression of colorectal cancer expressing S492R EGFR by monoclonal antibody CH12

Qiongna Dong, Bizhi Shi, Min Zhou, Huiping Gao, Xiaoying Luo, Zonghai Li, Hua Jiang

Journal Article

Fault tolerant control strategy for modular PWM current source inverter

Journal Article

Potential unreliability of ALK variant allele frequency in the efficacy prediction of targeted therapy in NSCLC

Journal Article

Engineering System of Weather Modification in China

He Zhijin,Wang Guanghe,Wang Yuzeng

Journal Article

Self-assembly of metal-cholesterol oxidase hybrid nanostructures and application in bioconversion of steroids derivatives

Yu Xin, Qiuyue Gao, Yu Gu, Mengyao Hao, Guangming Fan, Liang Zhang

Journal Article

UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY CROSSES WITH ‘ORAH’ MANDARIN (CITRUS RETICULATA)

Journal Article